PlantSPEAD (Plant Splicing-related Protein Expression and Annotation Database) shows a comprehensive presentation of pre-mRNA splicing-related proteins (SRPs), their annotations and expressions in model plants rice (Oryza sativa ssp. japonica) and Arabidopsis (Arabidopsis thaliana). The currently reported collection of 798 SRPs, 1,559 isoforms, 3,924 expression data, and 6,236 annotations identified to date. Our database is mainly divided into two categories; Monocot splicing-related proteins and Dicot splicing-related proteins.

The user can search the information through three approaches; quick search, advanced search and blast search. In the quick search, the user can see SRPs based on their classification. In the advanced search, some keywords including gene id, transcript id, gene name, chromosomal location, family/domain, splicing protein group, monocot/dicot specificity, rice/Arabidopsis specificity, GO term and protein domain database accession etc. can be used. In the blast search, blastp program enables the comparison between user’s peptide sequence and other biological sequences present in PlantSPEAD.

This is the most descriptive page about all the SRPs. The first table shows a quick summary of all SRPs with respect to monocot or dicot. This table can be scrolled down and navigated into next pages to access more SRPs. It possesses multiple columns including group of the splicing protein, gene id, transcript id, gene name, chromosome location, protein description, family/domain, remarks and reference. The user can click on either gene id or transcript id to browse further annotation and expression information of SRPs.

Plant mutants defective in splicing factors are severely impaired by abiotic stress. Therefore, the plant stress resistance improvement programmes, which focus on plant genome engineering can positively benefit from the content of PlantSPEAD. Our website and database encourage the user to identify, classify and compare the SRPs based on their annotation and expression. The PlantSPEAD provides a strong contribution to remove the knowledge gaps regarding SRPs in plants.